GeneXpert Testing

GeneXpert testing (commonly referred to as CBNAAT – Cartridge-Based Nucleic Acid Amplification Test) is a rapid molecular diagnostic test used primarily to detect tuberculosis (TB) and determine resistance to rifampicin, a key anti-TB drug.

What It Detects:

• Presence of Mycobacterium tuberculosis (MTB)
• Resistance to Rifampicin (marker of multidrug-resistant TB)

How It Works:

• A sputum sample or other body fluid (e.g., CSF, lymph node aspirate) is placed in a cartridge.
• The cartridge is loaded into the GeneXpert machine.
• The machine: Breaks down the bacteria, Extracts the DNA, Amplifies TB-specific genetic sequences, Detects rifampicin resistance mutations.
• Results are available in under 2 hours.

Advantages:

• Fast and highly accurate
• Detects even low numbers of bacteria
• Can be used on extra-pulmonary samples
• Simultaneously provides drug resistance information

Common Uses:

• Diagnosing pulmonary TB and extra-pulmonary TB
• Screening for drug-resistant TB
• Preferred test for children, HIV-positive patients, and critical cases
• WHO-recommended first-line test in many countries

Conclusion:

GeneXpert has revolutionized TB diagnosis by offering rapid, reliable, and early detection, especially crucial for initiating timely treatment and controlling drug-resistant TB spread. Meet Dr. Namrata Jasani GeneXpert Diagnostic Center in Byculla , Mumbai